Comparative bioinformatics analysis of the biosynthetic pathways and key candidate genes of three species, Vitis vinifera, Fragaria vesca and Olea europaea, furnish enzyme sets for the production of pharmaceutically valuable terpenes in heterologous hosts

Abstract Plants represent a rich repository of taxonomically restricted, yet chemically diverse, secondary metabolites that are synthesised via specific metabolic pathways. Enzyme specificity and biosynthetic gene clustering the bottleneck metabolite evolution. As economically important food crops, grape, strawberry, olive produce many pharmaceutically molecules; however, their pathways remain inaccessible. Our genomic-based analysis these three species reveal diversity specialised metabolites. We found over 20 BGCs predicted, most which were characterised in two species, grape strawberry. Gene annotation candidate genes predicted production medically industrially compounds including cycloartenol, nerolidol, farnesene valencene. Although clusters concentrated genomic positions, some have shown duplications clusters, is suggestive pseudogenes or misassembled genomes. genome mining putative functional annotated indicated evolutionary processes shaped current genetic structure structural chemical compositions. Revealing biogenetic background natural molecules step forward towards expansion diversification engineering heterologously, as well identification role interaction between those plants biotic abiotic conditions.